Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human NKG2-D Type ? Integral Membrane Protein (also known as KLRK1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human KLRK1 antibody. KLRK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human KLRK1 Antibody is added and binds to KLRK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KLRK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human KLRK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm